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Table 4 Neuraminidase activities of H9N2 AIV

From: Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens

virus K M (μM) a V max (fluorescence a.u/s) a Relative V max b Elution time(h)
rSD01 54.16 ± 3.12 192.73 ± 6.54 1.00 1.5
r01/NASS 49.167 ± 3.34 118.83 ± 1.43* 0.62 3.5
r01/NAHB 52.43 ± 6.68 123.83 ± 3.40* 0.64 2
r01/NA381 53.84 ± 2.53 92.25 ± 1.69* 0.48 4
  1. aThe enzyme kinetics data was fit to the Michaelis-Menten equation by nonlinear regression to determine the Michaelis constant (K M) and maximum velocity (Vmax) of substrate conversion. Results are given as the mean ± standard deviation from three duplicate samples. bRelative Vmax: homologous of recombinant virus to SD01. *P < 0.05 compared with SD01.