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Table 2 Number of chickens infected by recombinant virus in independent experiments*

From: Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens

Dpi rSD01 r01/NASS r01/NAHB r01/NA381 r01/NASS-381
Inoculated Direct-contact Aerosol-contact Inoculated Direct-contact Aerosol-contact Inoculated Direct-contact Aerosol-contact Inoculated Direct-contact Aerosol-contact Inoculated Direct-contact Aerosol-contact
2 6/10 2/10 0/10 5/10 0/10 0/10 5/10 2/10 0/10 0/10 0/10 0/10 3/10 0/10 0/10
4 10/10 10/10 6/10 10/10 5/10 0/10 10/10 10/10 3/10 10/10 2/10 0/10 10/10 6/10 2/10
6 10/10 10/10 8/10 10/10 9/10 0/10 10/10 10/10 8/10 10/10 8/10 0/10 10/10 9/10 5/10
8 9/10 9/10 10/10 6/10 6/10 0/10 8/10 7/10 10/10 8/10 4/10 0/10 10/10 10/10 7/10
10 9/10 7/10 9/10 4/10 3/10 0/10 8/10 5/10 7/10 4/10 2/10 0/10 8/10 7/10 6/10
12 7/10 6/10 7/10 2/10 0/10 0/10 4/10 2/10 3/10 1/10 0/10 0/10 6/10 5/10 4/10
14 5/10 3/10 6/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 3/10 2/10 0/10
  1. *In every independent experiment, ten SPF chickens (inoculated) were infected intranasally 107 EID50 of recombinant virus, then another ten animals (direct contact) were introduced into the same isolator 24 h later, while ten animals (aerosol contact) were placed separately in another isolator B. Oropharyngeal and cloacal swab samples of the chickens were collected at 2 day intervals and inoculated in SPF embryonated chicken eggs for observation virus shedding. The result expressed as infected number/total number of chickens. Repeat transmission experiments of r01/NASS and r01/NA381 which could not transmit by aerosols were performed. The results indicate that there was no virus shedding of all aerosol contact animals.