Figure 2

Human TLR2 response is mediated by H. bizzozzeronii LPS . (A) H. bizzozeronii LPS activates human TLR2 in dose dependent manner. HEK-Blue™-hTLR2 cells were incubated for 24 h with different concentrations of LPS from human isolate CIII-1^GEN (white bars) and canine isolate Heydar (grey bars) H. bizzozzeronii strains. HEK-Blue™-hTLR2 response decrease significantly (test for linear trend; p < 0.05). Error bars show ± SEM. (B) Inhibitory effect of Polymyxin B in TLR2 activation by H. bizzozzeronii LPS. LPS (12.5 μg.mL⁻¹) extracted from the human strain CIII-1^GEN (white bars) and the canine Heydar (grey bars) H. bizzozzeronii strains, were pre-incubated with PB (40 μg.mL⁻¹) for 45 min at 37 °C. HEK-Blue™-hTLR2 cells were incubated with LPS (with and without PB treatment) for 24 h. HEK-Blue™-hTLR2 response was inhibited in both strains after treatment with PB and the response was significantly different (p < 0.05) in both the strains. The values were normalized by subtracting the average OD₆₂₀ values of the untreated cells. The OD₆₂₀ value of the positive control Pam2CSK4 was 2.25 ± 0.12. Error bars show ± SEM.