Differential human TLR2 activation by
LPS. HEK-Blue™-hTLR2 cells were incubated for 24 h with LPS (25 μg.mL−1) extracted from different human (white bars) and canine (grey bars) strains, and NF-κB-induced SEAP activity was assessed using QUANTI-Blue™ and by reading the OD at 620 nm. The values were normalized by subtracting the average OD620 values of the untreated cells. The OD620 value of the positive control Pam2CSK4 was 2.25 ± 0.12. Error bars show ± SEM.