Table 1 PCR primers used for the amplification of B. hyodysenteriae plasmid genes not on the GeneChip
Primer namea | Sequence (5′ – 3′) | Product size |
|---|---|---|
BHWA1_02666a-F | gagaagaatatttaatacctttaatag | 217 bp |
BHWA1_02666a-R | cattcatatatataataataatggttgg | |
BHWA1_02666b-F | ccaaccattattattatatatatgaatg | 549 bp |
BHWA1_02666b-R | ctaaatctctatattgtttaactatag | |
BHWA1_02666c-F | ctatagttaaacaatatagagatttag | 177 bp |
BHWA1_02666c-R | ctatttttatacctaatatggtgaatat | |
BHWA1_02667a-F | actggagttgctggatttataggatc | 560 bp |
BHWA1_02667a-R | aagtcaggtctctgtctctttcc | |
BHWA1_02667b-F | caaataaagatcatactgttataggaatag | 597 bp |
BHWA1_02667b-R | atgtatagtcacgcatagtgg | |
BHWA1_02667c-F | tgtaatacatttagcaggatatgg | 384 bp |
BHWA1_02667c-R | ggtataggattattttcaagtatcag | |
BHWA1_02668a-F | gttcataccatttagaaaaagaagag | 701 bp |
BHWA1_02668a-R | gttcataccatttagaaaaagaagag | |
BHWA1_02668b-F | agaacaaaacaacataaagcatc | 206 bp |
BHWA1_02668b-R | catcagtaaaacaaatataatccc | |
BHWA1_02668c-F | cctgagcattatggactttc | 240 bp |
BHWA1_02668c-R | tgtactgtctgattttttatggtc | |
BHWA1_02672a-F | aaatgtagaagatattgtattgcc | 417 bp |
BHWA1_02672a-R | acctctcctatatgttttttatacttag | |
BHWA1_02672b-F | attactacaaaatgtactctaaaatgtaag | 546 bp |
BHWA1_02672b-R | ccatactatatgacaaaaataaaatctag | |
BHWA1_02672c-F | tatctaagtataaaaaacatataggagagg | 498 bp |
BHWA1_02672c-R | cagcacaaaactcacatagtg | |
BHWA1_02673a-F | aaatacttgtcaataatcttagtgg | 1819 bp |
BHWA1_02673a-R | tttcatcataagcaaaaataatatc | |
BHWA1_02673b-F | gtaagtggaaaaagaatgaaacatac | 1032 bp |
BHWA1_02673b-R | agattgtcttgacgaataaaag | |
BHWA1_02673c-F | aataaatatgacattaaaggaataaaaatc | 805 bp |
BHWA1_02673c-R | ctattgttagtagcaaaataataaaaatac | |
BHWA1_02674a-F | atttagaagatgtaatacctttagagg | 249 bp |
BHWA1_02674a-R | tcattttcgctatatttttatttac | |
BHWA1_02674b-F | ttatacaaaataggagagcctttag | 363 bp |
BHWA1_02674b-R | atcgcaataatctgaaaatg | |
BHWA1_02674c-F | gtatgtacttatcttttttattctattgtc | 194 bp |
BHWA1_02674c-R | catattggatttttatctctatgtc | |
BHWA1_02675a-F | attggatagaacatagagggag | 301 bp |
BHWA1_02675a-R | actgtatcatttgctatttcattag | |
BHWA1_02675b-F | tataaaaactataagaatatctctacaagg | 367 bp |
BHWA1_02675b-R | aacatataaggtataaaatggttgag | |
BHWA1_02675c-F | cctcaaccattttataccttatatg | 184 bp |
BHWA1_02675c-R | taactatattttctcgttttccttg |