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Table 1 Antibodies used for flow cytometry analyses

From: Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome

Antigen Clone Isotype Source Fluorochrome Secondary Ab
T-helper cells
CD3 BB23-8E6-8C8 IgG2a BD Biosciences PerCP-Cy5.5  
CD4 74-12-4 IgG2b BD Biosciences FITC  
CD8α 76-2-11 IgG2a BD Biosciences Alexa647  
Cytolytic T cells
CD3 BB23-8E6 IgG2b Southern Biotech FITC  
CD8β PPT23 IgG1 In-house   goat anti-mouse IgG1, Alexa647 (Invitrogen)
SLA-DR MSA3 IgG2a In-house   goat anti-mouse IgG2a, PE (Southern Biotech)
γδ T cells
Pan-γδ PPT16 IgG2b In-house   goat anti-mouse IgG2b, Alexa488 (Invitrogen)
CD2 RPA-2.10 IgG1 AbD Serotec PE  
CD8α 76-2-11 IgG2a BD Biosciences Alexa647  
NK cells
CD3 BB23-8E6-8C8 IgG2a BD Biosciences PerCP-Cy5.5  
CD8α 76-2-11 IgG2a BD Biosciences Alexa647  
B cells   
CD21 B-ly4 IgG1 BD APC  
CD79α HM57 IgG1 Dako PE  
Monocytes
CD172a 74-22-15 IgG1 Southern Biotech PE  
CD4 74-12-4 IgG2b BD Biosciences Alexa647  
CD14 MIL-2 IgG2b AbD Serotec FITC