Table 1 Antibodies used for flow cytometry analyses
Antigen | Clone | Isotype | Source | Fluorochrome | Secondary Ab |
|---|---|---|---|---|---|
T-helper cells | |||||
CD3 | BB23-8E6-8C8 | IgG2a | BD Biosciences | PerCP-Cy5.5 | |
CD4 | 74-12-4 | IgG2b | BD Biosciences | FITC | |
CD8α | 76-2-11 | IgG2a | BD Biosciences | Alexa647 | |
Cytolytic T cells | |||||
CD3 | BB23-8E6 | IgG2b | Southern Biotech | FITC | |
CD8β | PPT23 | IgG1 | In-house | goat anti-mouse IgG1, Alexa647 (Invitrogen) | |
SLA-DR | MSA3 | IgG2a | In-house | goat anti-mouse IgG2a, PE (Southern Biotech) | |
γδ T cells | |||||
Pan-γδ | PPT16 | IgG2b | In-house | goat anti-mouse IgG2b, Alexa488 (Invitrogen) | |
CD2 | RPA-2.10 | IgG1 | AbD Serotec | PE | |
CD8α | 76-2-11 | IgG2a | BD Biosciences | Alexa647 | |
NK cells | |||||
CD3 | BB23-8E6-8C8 | IgG2a | BD Biosciences | PerCP-Cy5.5 | |
CD8α | 76-2-11 | IgG2a | BD Biosciences | Alexa647 | |
B cells | |||||
CD21 | B-ly4 | IgG1 | BD | APC | |
CD79α | HM57 | IgG1 | Dako | PE | |
Monocytes | |||||
CD172a | 74-22-15 | IgG1 | Southern Biotech | PE | |
CD4 | 74-12-4 | IgG2b | BD Biosciences | Alexa647 | |
CD14 | MIL-2 | IgG2b | AbD Serotec | FITC | |