Changes in γδ T cells in response to PRRSv infection in pregnant gilts. A) Dot plots: The gating strategy for γδ T cells and CD2/CD8α defined subpopulations is demonstrated using representative data from gilt #53. B-E) Line charts: Changes in absolute numbers (mean ± SD) of total γδ T cells (blue), CD2−CD8α− γδ T cells (orange), CD2−CD8α+ γδ T cells (purple), and CD2+CD8α+ γδ T cells (green) are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days. F-H) Bar charts: The mean percentages (+SD) of CD2−CD8α− γδ T cells (orange), CD2−CD8α+ γδ T cells (purple), and CD2+CD8α+ γδ T cells (green) within total γδ T cells from INOC and CTRL gilts are presented for the respective study days. Superscript letters indicate significant differences (P < 0.01) between study days within INOC or CTRL gilts.