Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile

Table 2 Percentage of lymphocyte subsets in the milk from healthy and bovine leukemia virus (BLV)-infected cows

Group/Variables	Negative ( n = 25)	AL ( n = 16)	PL ( n = 17)
CD3 ⁺ (T cells) (%)	7.13 ± 5.04^a	10.46 ± 9.30^a	11.66 ± 7.27^a
CD4 ⁺ /CD8 ⁻ T cells (%)	1.32 ± 1.19^a	1.83 ± 1.73^a	2.55 ± 2.48^a
CD4 ⁻ /CD8 ⁺ T cells (%)	3.15 ± 2.57^a	4.45 ± 2.02^a	4.51 ± 3.86^a
CD4 ⁺ /CD8 ⁺ T cells (%)	0.41 ± 0.36^a	0.33 ± 0.30^a	0.23 ± 0.19^a
CD4 ⁻ /CD8 ⁻ T cells (%)	2..24 ± 2.33^a	3.84 ± 5.74^a	4.38 ± 3.87^a
CD3 ⁺ (T cells) (%)	6.31 ± 4.99^a	10.28 ± 10.44^a	11.06 ± 6.81^a
CD4 ⁺ /CD25 ⁻ T cells (%)	1.38 ± 1.32^a	1.84 ± 1.67^a	2.70 ± 2.46^a
CD4 ⁻ /CD25 ⁺ T cells (%)	0.06 ± 0.04^a	0.13 ± 0.18^a	0.15 ± 0.14^a
CD4 ⁺ /CD25 ⁺ T cells (%)	0.24 ± 0.23^a	0.21 ± 0.29^a	0.29 ± 0.36^a
CD4 ⁻ /CD25 ⁻ T cells (%)	4.63 ± 4.11^a	8.11 ± 8.58^a	7.92 ± 4.62^a
CD21 ⁺ (B cells) (%)	7.90 ± 5.53^a	13.02 ± 8.14^a	17.44 ± 7.20^a
CD5 ⁺ /CD11 ⁻ B cells (%)	0.87 ± 0.88^a	0.71 ± 0.45^a	0.72 ± 0.47^a
CD5 ⁻ /CD11 ⁺ B cells (%)	3.84 ± 4.01^a	6.80 ± 6.97^a	8.59 ± 5.15^a
CD5 ⁺ /CD11 ⁺ B cells (%)	0.85 ± 0.78^a	0.85 ± 0.51^a	1.01 ± 0.66^a
CD5 ⁻ /CD11 ⁻ B cells (%)	2.35 ± 1.73^a	4.67 ± 3.26^b	7.11 ± 3.89^c

Different superscripted letters^a,b,c within a row indicate significant differences (P ≤ 0.05) between the values. The results are shown as the mean ± SD.
AL: aleukemic BLV-infected cows; PL: BLV-infected cows with persistent lymphocytosis.

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