Table 1 Bacterial strains, plasmids and primers used in this study

Plasmids^a

pEP4351

Ori R6K dependent protein pir,; RP4 oriT; Cm^r Tc^r (Em^r); Tn4351 vector transfer.

[39]

pCP23

ColE1 ori, (pCP1 ori), Ap^r (Tc^r), E. coli–F. psychrophilum shuttle plasmid

[40]

pCP23-fpgA

FP1638 gene, derived from pCP23.

This study

pCP23-Gfpp2

pCP23-G carrying fpp2-fpp1 promoter

[41]

Bacterial strains

F. psychrophilum

THC02/90

Wild-type.

[42]

fpgA⁻

FP1638 mutant created by Tn4351 transposition.

This study

fpgA+

Mutant ΔFP1638 carrying pCP23-FP1638 plasmid, complemented strain.

This study

THC02/90-G

THC02/90 strain carrying pCP23-G plasmid

[41]

THC02/90 -fpp2

THC02/90 strain carrying pCP23-Gfpp2 plasmid

[41]

fpgA⁻ -fpp2

ΔFP1638 strain carrying pCP23-Gfpp2 plasmid

[41]

E. coli

S17-1 λpir

λpir hsdR pro thi; RP4-2 Tc::Mu Km::Tn7

[34]

BW19851

RP4-2tet::Mu-1kan::Tn7 integrant; _uidA::pir_ recA1 hsdR17 creB510 endA1 zbf-5 thi

[35]

Primers^b

26-F

5′ACTGGGATCCAGTTTTAAGCCCGCAAA 3′

This study

26-R

5′ ACTGCTGCAGCAATGAACTTCGTCTTG 3′

This study

TN-1

5′ GGACCTACCTCATAGACAA 3′

[19]

IS4351-F

5′ TCAGAGTGAGAGAAAGGG 3′

[19]

promfpp2-F

5′ ATCAGGATCCGAGCACTACACTTTCTAGA 3′

[41]

promfpp2-R

5′ GATTGGATCCTGTTCGGTAGTGTAGCA 3′

[41]