Plasmidsa
| | |
pEP4351
|
Ori R6K dependent protein pir,; RP4 oriT; Cmr Tcr (Emr); Tn4351 vector transfer.
|
[39]
|
pCP23
|
ColE1 ori, (pCP1 ori), Apr (Tcr), E. coli–F. psychrophilum shuttle plasmid
|
[40]
|
pCP23-fpgA
|
FP1638 gene, derived from pCP23.
|
This study
|
pCP23-Gfpp2
|
pCP23-G carrying fpp2-fpp1 promoter
|
[41]
|
Bacterial strains
| | |
F. psychrophilum
| | |
THC02/90
|
Wild-type.
|
[42]
|
fpgA−
|
FP1638 mutant created by Tn4351 transposition.
|
This study
|
fpgA+
|
Mutant ΔFP1638 carrying pCP23-FP1638 plasmid, complemented strain.
|
This study
|
THC02/90-G
|
THC02/90 strain carrying pCP23-G plasmid
|
[41]
|
THC02/90 -fpp2
|
THC02/90 strain carrying pCP23-Gfpp2 plasmid
|
[41]
|
fpgA− -fpp2
|
ΔFP1638 strain carrying pCP23-Gfpp2 plasmid
|
[41]
|
E. coli
| | |
S17-1 λpir
|
λpir hsdR pro thi; RP4-2 Tc::Mu Km::Tn7
|
[34]
|
BW19851
|
RP4-2tet::Mu-1kan::Tn7 integrant; _uidA::pir_ recA1 hsdR17 creB510 endA1 zbf-5 thi
|
[35]
|
Primersb
| | |
26-F
|
5′ACTGGGATCCAGTTTTAAGCCCGCAAA 3′
|
This study
|
26-R
|
5′ ACTGCTGCAGCAATGAACTTCGTCTTG 3′
|
This study
|
TN-1
|
5′ GGACCTACCTCATAGACAA 3′
|
[19]
|
IS4351-F
|
5′ TCAGAGTGAGAGAAAGGG 3′
|
[19]
|
promfpp2-F
|
5′ ATCAGGATCCGAGCACTACACTTTCTAGA 3′
|
[41]
|
promfpp2-R
|
5′ GATTGGATCCTGTTCGGTAGTGTAGCA 3′
|
[41]
|