Ten dead, 6-shape to comma-shaped Trichinella larvae were picked randomly and transferred to approximately 2 mL tap water. Subsequently, the larvae were rinsed into 2 liter of meat digest fluid. The spiked fluid was either transferred to a separatory funnel and left to sediment for 30 min according to EU 2075/2005, or passed through a stack of stainless steel sieves according to SSM. SSM performed significantly better than EU-RM for detection of dead larvae in meat digest (p =6 · 10-12, Fisher’s Exact test).
* # of larvae found after sieving the residual fluids through 38 μm sieve following sedimentation.