Skip to main content

Table 1 Recovery of dead Trichinella larvae spiked in meat digest

From: Trend analysis of Trichinella in a red fox population from a low endemic area using a validated artificial digestion and sequential sieving technique

   EU 2075 2005 SSM
  Spike Sedimentation Residual fluids* 63 μm 38 μm
1 10 4 6 8 0
2 10 7 3 8 1
3 10 2 8 9 0
4 10 4 5 10 0
5 10 6 3 10 0
6 10 9 1 10 0
7 10 6 3 8 0
8 10 8 2 10 0
9 10 8 2 10 0
10 10 6 4 9 0
sum: 100 60 37 92 1
  1. Ten dead, 6-shape to comma-shaped Trichinella larvae were picked randomly and transferred to approximately 2 mL tap water. Subsequently, the larvae were rinsed into 2 liter of meat digest fluid. The spiked fluid was either transferred to a separatory funnel and left to sediment for 30 min according to EU 2075/2005, or passed through a stack of stainless steel sieves according to SSM. SSM performed significantly better than EU-RM for detection of dead larvae in meat digest (p =6 · 10-12, Fisher’s Exact test).
  2. * # of larvae found after sieving the residual fluids through 38 μm sieve following sedimentation.