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Table 1 Recovery of dead Trichinella larvae spiked in meat digest

From: Trend analysis of Trichinella in a red fox population from a low endemic area using a validated artificial digestion and sequential sieving technique

  

EU 2075 2005

SSM

 

Spike

Sedimentation

Residual fluids*

63 μm

38 μm

1

10

4

6

8

0

2

10

7

3

8

1

3

10

2

8

9

0

4

10

4

5

10

0

5

10

6

3

10

0

6

10

9

1

10

0

7

10

6

3

8

0

8

10

8

2

10

0

9

10

8

2

10

0

10

10

6

4

9

0

sum:

100

60

37

92

1

  1. Ten dead, 6-shape to comma-shaped Trichinella larvae were picked randomly and transferred to approximately 2 mL tap water. Subsequently, the larvae were rinsed into 2 liter of meat digest fluid. The spiked fluid was either transferred to a separatory funnel and left to sediment for 30 min according to EU 2075/2005, or passed through a stack of stainless steel sieves according to SSM. SSM performed significantly better than EU-RM for detection of dead larvae in meat digest (p =6 · 10-12, Fisher’s Exact test).
  2. * # of larvae found after sieving the residual fluids through 38 μm sieve following sedimentation.