Figure 3

Quantitative measurements of the sensitivity and permissivity of carp to CyHV-3 during the early stages of development. Fish were mock-inoculated or inoculated at the indicated times post-hatching and then analyzed by IVIS at 24 and 72 hpi. (a) The average radiance (p/sec/cm²/sr) emitted by individual infected fish (n?=?30 per group) corrected for the background of each image is presented by dots. For each group of inoculated fish (according to time of inoculation and time post-inoculation), the mean?+?SD is presented as grey bars. For each time point, a group of mock-inoculated fish was analyzed to define the threshold of positivity (green bars), defined as the mean?+?3 SD (p?<?0.00135). Red dots represent infected fish defined as positive based on their average radiance being higher than the threshold or based on the expression of a spot of light (even if their average radiance was below the threshold). The data obtained at different times post-inoculation (24 versus 72 hpi) for each time of inoculation were compared by two-way ANOVA. (b) The number of positive fish among 30 analyzed inoculated fish. The number of positive fish according to time of inoculation was compared by logistical analysis. Inoculations performed at the embryonic and larval stages (between 0 and 14 days post-hatching) were compared to inoculations at the post-larval stages (between 21 and 35 days post-hatching). *p?<?0.05, ****p?<?0.0001.