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Table 1 RNA strand specific real-time RT-PCR

From: Low virulent infectious salmon anaemia virus (ISAV) replicates and initiates the immune response earlier than a highly virulent virus in Atlantic salmon gills

 

Gill mRNA

Gill cRNA

Kidney mRNA

Kidney cRNA

Heart mRNA

Heart cRNA

Day

LVI

HVI

LVI

HVI

LVI

HVI

LVI

HVI

LVI

HVI

LVI

HVI

0.25

0

0

0

0

0

0

0

0

na

na

na

na

1

4

0

1

0

0

0

0

1

0

0

0

0

2

4

0

2

0

0

0

0

0

1

0

0

0

3

4

0

3

1

4

2

1

0

3

0

1

0

4

4

3

2

1

4

4

3

3

4

1

2

2

5

4

3

3

2

4

3

4

3

4

2

4

3

6

4

4

4

2

4

3

4

3

4

3

4

3

7

4

4

4

3

4

4

4

4

4

4

4

4

8

4

4

4

2

4

4

4

4

na

na

na

na

10-23

4

4

4

4

4

4

4

4

na

na

na

na

  1. Number of fish (max. = 4) testing positive for mRNA or cRNA of low virulent (LVI) or highly virulent (HVI) virus using RNA strand specific real-time RT-PCR analysis of gill, anterior kidney and heart. na = not applicable.