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Figure 5 | Veterinary Research

Figure 5

From: Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain

Figure 5

Effects of TLR3 mRNA knockdown on sELR1 and INFβ expression in eMDM. (A) Knockdown of TLR3 expression in eMDM with siRNA. At 48 h after transfection with siRNA targeting equine TLR3 (siRNA-TLR3), the expression level of TLR3 mRNA was quantified with real-time qPCR. siRNA with a scrambled sequence (siRNA-C) was used as the control. (B) The induction of TLR3 expression by poly I:C in siRNA-TLR3-transfected eMDM. The cells were transfected with siRNA-TLR3 or siRNA-C. TLR3 mRNA was measured with real-time qPCR 12 h after stimulation with 0.5 μg/mL poly I:C. (C) Kinetics of TLR3, sELR1 and INFβ mRNA expression induced by EIAVFDDV13 in eMDM transfected with siRNA-TLR3. Cells were transfected with siRNA-TLR3 or siRNA-C. After 48 h of incubation, EIAVFDDV13 was added to the cells, and total RNA was extracted at different times after infection. The mRNA copies were specifically amplified and quantified with bDNA (for TLR3 and INFβ) and qPCR (for sELR1). The values of Y axis were treated by Log2. *P < 0.05.

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Veterinary Research

ISSN: 1297-9716