Figure 2

Immunization of chickens with recombinant proteins. Chickens were immunized once with 100 μg recombinant protein (GtxA-N, FlfA, Gab_1309, Gab_2156, or Gab_2312) or a placebo (non-immunized), followed by intraperitoneal challenge with G. anatis 12656–12. The scoring of the lesions found in the peritoneum was done according to three parameters: (i) inflammatory reaction, (ii) amount of exudate, and (iii) clarity of the peritoneal surfaces. All parameters were scored on a scale from 0–3, thus giving a maximum score of 9. The horizontal lines shows the group median, and the difference between the lesions scores of immunized and non-immunized groups was analyzed using Mann Whitney U test. Asterisks indicate statistical significance (*P < 0.05).