Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle

Bihannic, Morgan; Ghanbarpour, Reza; Auvray, Frédéric; Cavalié, Laurent; Châtre, Pierre; Boury, Michèle; Brugère, Hubert; Madec, Jean-Yves; Oswald, Eric

doi:10.1186/s13567-014-0076-9

Veterinary Research

Table 2 Oligonucleotide primers used for detection of the virulence-associated genes on E . coli isolates from feces of calves with diarrhea in Iran

From: Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle

Gene/DNA fragment	Primer sequence (5′ - 3′)	Product size (bp)	Reference
aap	CTTGGGTATCAGCCTGAATG	310	[22]
aap	AACCCATTCGGTTAGAGCAC	310	[22]
aggR	CTAATTGTACAATCGATGTA	457	[22]
aggR	AGAGTCCATCTCTTTGATAAG	457	[22]
AA probe	CTGGCGAAAGACTGTATCAT	629	[22]
AA probe	CAATGTATAGAAATCCGCTGTT	629	[22]
f17A family	GCAGAAAATTCAATTTATCCTTGG	537	[10]
f17A family	CTGATAAGCGATGGTGTAATTAAC (P2)*	537	[10]
f17Aa	GCTGGAAGGGTGCAATACGCCTG	321	[10]
f17Ab	CAACTAACGGGATGTACAGTTTC	323	[10]
f17Ac	GCAGGAACCGCTCCCTTGGC	416	[10]
f17Ad	GATAGTCATAACCTTAATATTGCA	239	[10]
f17G1	CGGAGCTAATACTGCATCAACC	615	[10]
f17G1	TGTTGATATTCCGTTAACCGTAC	615	[10]
f17G2	CGTGGGAAATTATCTATCAACG	615	[10]
f17G2	TGTTGATATTCCGTTAACCGTAC	615	[10]
afa E-VIII	CTAACTTGCCATGCTGTGACAGTA	302	[23]
afa E-VIII	TTATCCCCTGCGTAGTTGTGAATC	302	[23]
f5	TATTATCTTAGG TGGTATGG	314	[24]
f5	GGTATCCTTTAGCAGCAGTATTTC	314	[24]
f41	GCATCAGCGGCAGTATCT	380	[24]
f41	GTCCCTAGCTCAGTATTATCACCT	380	[24]
stx1	AGAGCGATGTTACGGTTTG	388	[25]
stx1	TTGCCCCCAGAGTGGATG	388	[25]
stx2	TGGGTTTTTCTTCGGTATC	807	[25]
stx2	GACATTCTGGTTGACTCTCTT	807	[25]
eaeA	AGGCTTCGTCACAGTTG	570	[25]
eaeA	CCATCGTCACCAGAGGA	570	[25]
stI	ATTTTTMTTTCTGTATTRTCTT	190	[26]
stI	CACCCGGTACARGCAGGATT	190	[26]
ltI	GGCGACAGATTATACCGTGC	450	[26]
ltI	CGGTCTCTATATTCCCTGTT	450	[26]
ipaH	GTTCCTTGACCGCCTTTCCGATACCGTC	600	[26]
ipaH	GCCGGTCAGCCACCCTCTGAGAGTAC	600	[26]
papE-F	GCAACAGCAACGCTGGTTGCATCAT	336	[27]
papE-F	AGAGAGAGCCACTCTTATACGGACA	336	[27]
afaI B-C	GCTGGGCAGCAAACTGATAACTCTC	750	[27]
afaI B-C	CATCAAGCTGTTTGTTCGTCCGCCG	750	[27]
sfa/focDE	CGGAGGAGTAATTACAAACCTGGCA	410	[27]
sfa/focDE	CTCCGGAGAACTGGGTGCATCTTAC	410	[27]
hly	AACAAGGATAAGCACTGTTCTGGC	1177	[27]
hly	ACCATATAAGCGGTCATTCCCGTCA	1177	[27]
iucD	TACCGGATTGTCATATGCAGACCG	602	[27]
iucD	AATATCTTCCTCCAGTCCGGAGAAG	602	[27]
cnf1	GGGGGAAGTACAGAAGAATTA	1111	[28]
cnf1	TTGCCGTCCACTCTCACCAGT	1111	[28]
cnf2	TATCATACGGCAGGAGGAAGCACC	1240	[28]
cnf2	GTCACAATAGACAATAATTTTCCG	1240	[28]
cdtIII	GAAAATAAATGGAATATAAATGTCCG	555	[28]
cdtIII	TTTGTGTCGGTGCAGCAGGGAAAA	555	[28]
cdtIV	CCTGATGGTTCAGGAGGCTGGTTC	350	[28]
cdtIV	TTGCTGCAGAATCTATACCT	350	[28]
clpG	GGGCGCTCTCTCCTTCAAC	402	[29]
clpG	CGCCCTAATTGCTGGCGAC	402	[29]
chuA	GACGAACCAACGGTCAGGAT	279	[30]
chuA	TGCCGCCAGTACCAAAGACA	279	[30]
yjaA	TGAAGTGTCAGGAGACGCTG	211	[30]
yjaA	ATGGAGAATGCGTTCCTCAAC	211	[30]
TspE4.C2	GAGTAATGTCGGGGCATTCA	152	[30]
TspE4.C2	CGCGCCAACAAAGTATTACG	152	[30]

*P2 was used as common reverse primer for f17A family, f17Aa, f17Ab, f17Ac, f17Ad detection.

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ISSN: 1297-9716

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