Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Table 1 Primers used in this study ^a

Name	Sequence (5′-3′)	Position	Target gene
N-F	5′ GGGACAAGTGAAAATGTCTCTTCAGGGGATTCA GCTTA 3′	41-77	N
N-R	5′ TTTTTAATTACTCATAATCATTCTGGCCT 3′	1235-1263	N
P-F	5′ GGGACAAGTCAAAATGTCCTTTCC 3′	1248-1271	P
P-R	5′ GTTTTTTATTAACTACATAGTAAGGGAGTATAG GTCATC 3′	2136-2174	P
M-F	5′ GGGGACAAGTIAAIATGGAGTC 3′	2157-2175	M
M-R	5′ GTCTTGGCTATCGCTACACC3′	3399-3418	M
F-F	5′ GGGACAAGTGAAAATGTCTTGG 3′	3208-3229	F
F-R	5′ TCTTCACTTGTCCCAATTTTTT 3′	4666-4687	F
M2SH-F	5′ TTGGGACAAGTGAAGATGTCTCG 3′	4672-4694	M2-SH
M2SH-R	5′ CTTGACTTTGACTTTAAGCTCCTG 3′	6186-6209	M2-SH
L1-F	5′ GGACCAAGTTAAAAATGGATCCAC 3′	7939-7962	L
L1-R	5′ TACACTCCTTCTGTTTCTGGAGG 3′	10006-10028	L
L2-F	5′ TGGCTGCATTTGACTGTTCCACT 3′	9951-9983	L
L2-R	5′ CACTAAGTCCAATTTGTCAGGG 3′	12068-12089	L
L3-F	5′ TACAGGCAGAAGCCCTAAACAAT 3′	11933-11955	L
L3-R	5′ GCAAAAAAACCGTATTCATCC 3′	14126-14146	L
G-F	5′ CAGCCAGGCAATCACACAACAGTTC 3′	5881-5904	G
G-R	5′ CCACTTTCGAAGTGTTATCCTTTTT 3′	8076-8099	G

^aPrimers according to available subgroup C aMPV Colorado strain, GenBank accession number: AY590688.

ISSN: 1297-9716