LPS characterization. Tricine SDS-PAGE (A) and Western-blot (B) analysis of the LPS of B. ovis PA (1) and the LPS mutants BoΔwadA (2), BoΔwadB (3) and BoΔwadC (4). Arrows point the components I, II and III found by migration in the gel. The antibody used in the blot shown (B) is anti R-LPS A68/10A06/B11. Western blot probed with A68/24G12/A08, and A68/24D08/G09 gave similar results (data not shown).