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Figure 7 | Veterinary Research

Figure 7

From: Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle

Figure 7

The Tp5 87-95 epitope is presented by two BoLA molecules (BoLA-T5 and BoLA-1*02301). Panel A: predicted binding affinity of Tp587-95 peptide epitope for BoLA-1*02301 and BoLA-T5; Panel B: The cell line BV50 stained with anti-bovine CD8 (PerCP) and Tp587-95 p-MHC class I tetramers made with BoLA-1*02301 or BoLA-T5. Panel C: The cell line BV50 co-stained with anti-bovine CD8 (PerCP), Tp587-95-BoLA-1*02301 tetramers (PE) and Tp587-95-BoLA-T5 tetramers (APC). Tetramer labelling was measured on the CD8-gated population.

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