Multiple staining of Tp1
peptide-MHC tetramer positive cells. In each panel the left graph represents unstained negative control cells. The cell line BB007 was stained with Tp1214-224-BoLA-6*01301 tetramer in conjunction with anti-bovine CD8 (panel A), anti-Fas-ligand (panel B), or anti-perforin (panel C). Panel D: BB007 CTL line stained with Tp1214-224-BoLA-6*01301 tetramer and with anti-bovine CD8 and anti-perforin. Data in Panels A, B and C, and data in Panel D are from two independent experiments.