Figure 1

Tp1 214-224 -BoLA-6*01301 p-MHC class I tetramer staining of bovine CTL line BB007. The CTL line was stained with buffer alone (panel A), p-MHC class I tetramers made with the control peptide, YMYRVWSPL (panel B), p-MHC class I tetramers made with the peptide Tp296-104, which is restricted by another BoLA molecule [BoLA-T2c] (panel C), p-MHC class I tetramers made in the absence of peptide (panel D) and p-MHC class I tetramers made with peptide Tp1214-224 (panel E). All tetramers were generated with PE-streptavidin.