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Table 2 Detection of SBV RNA in IFNAR -/- mice inoculated with 100 μL of semen from bull 7 (Experiments 1 and 2) or with SBV RNA positive blood from IFNAR -/- mice (Experiment 3)

From: Evidence of excretion of Schmallenberg virus in bull semen

Inoculum

Exp. N°

Mice N°

Ctinoculum

Ctblood (4 days pi)

ELISA results

Bull 7 (24/08/2012)

2

8587

33.6

36.7

Neg

8585

Neg

Neg

8589

24.0

Neg

8581

Neg

Neg

Bull 7 (29/08/2012)

1

7728

17.1

28.9

Pos

8216

23.5

Pos

8410

23.0

Pos

2

8583

19.7

21.7

Pos

8849

20.0

Pos

8582

25.6

Pos

8588

26.0

Pos

Bull 7 (07/09/2012)

2

8827

22.3

31.5

Pos

8571

Neg

Neg

8847

Neg

Neg

8856

37.0

Pos

Bull 7 (17/09/2012)

2

8621

23.1

Neg

Neg

8709

Neg

Neg

8853

Neg

Neg

8630

Neg

Neg

Inoculum A (1/10)

3

8631

22.0

22.1

Pos

8634

Neg

Neg

8858

Neg

Neg

8629

Neg

Neg

Inoculum A (1/100)

3

8688

25.0

Neg

Neg

8682

Neg

Neg

8848

Neg

Neg

8625

Neg

Neg

  1. qRT-PCR results from 10 μL of straws or blood sampled from IFNAR-/- mice, 4 days pi.
  2. Bold data correspond to samples with a confirmed detection of SBV RNA (Ct inoculum and blood) or positive ELISA results.