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Table 1 Bovine viral diarrhoea virus (BVDV) detection and pathology in rabbits at day 5 after challenge by intravenous route (IV) or nebulizer (N)

From: Experimental infection of rabbits with bovine viral diarrhoea virus by a natural route of exposure

  BVDV RT-PCRa Virus isolation GALT depletionc
Animal Buffy coat Ileum Appendix Spleen Ileum  
IV5 neg + + + + moderate-severe
IV6 + + + + + mild-moderate
IV7 + + + + + moderate-severe
IV8b neg neg neg neg neg none
N5 neg + + + not done indeterminatec
N6 neg neg + + not done mild-moderate
N7 neg neg neg + not done mild-moderate
N8b neg neg neg neg not done indeterminatec
  1. aRT-PCR for viral RNA detection was performed as described in the materials and methods. In each case, 1 μL of total RNA was used in assays specific for BVDV type 1 and for genomic β-actin. Assays with Ct for BVDV and for actin less than 40 were considered positive (+), while assays with Ct for BVDV greater than 40.1 and Ct for actin less than 40 were considered negative (neg).
  2. bnegative control animals.
  3. cGut associated lymphoid tissue (GALT) depletion was assessed by histopathology as described in the materials and methods section. In group N, GALT depletion could not be determined clearly in two animals due to autolysis.