E. tenella antigen-specific T
1 and T
2 cytokine-secreting cells quantified by ELISPOT assay. Samples were taken from the spleen, cecal tonsils, and Peyer’s patches at 6 dpi. Each bar represents the mean ± SD value from three independent experiments. Within each graph, bars with different letters are significantly different (P < 0.05) according to Duncan’s multiple-range test.