Early changes in cytokine expression in peste des petits ruminants disease

Table 2 PCR primer pairs and reaction conditions used in the work described in this paper

Target	Primer sequences	Ta¹	[Primer]²	Reference
IL-1β	CCTTGGGTATCAGGGACAA	60	200 nM	This paper
	GGGTATGGCTTTCTTTAGG
IL-4	ACCTGTTCTGTGAATGAAGCCAA	61	300 nM	This paper
	CTTCATAATAGTCTTTAGCCTTTC
IL-6	TCCAGAACGAGTTTGAGG	60	400 nM	[18]
	CATCCGAATAGCTCTCAG
IL-8	ATGAGTACAGAACTTCGA	52	300 nM	[18]
	TCATGGATCTTGCTTCTC
IL-10	TGCTGTTGACCCAGTCTCTG	60	200 nM	[17]
	AGGGCAGAAAACGATGACAG
IL-12A	GGGCATTGTCTGGCTTCTG	60	200 nM	This paper
	TTCTTCCAGGGAGGGTTTCT
IL-12B	GCTGGGAGTACCCTGACACG	63	200 nM	[19]
	GTGACTTTGGCTGAGGTTTGGTC
IL-18	ACTGTTCAGATAATGCACCCCAG	60	300 nM	[19]
	TTCTTACACTGCACAGAGATGGTTAC
Interferon β	CCAGATGGTTCTCCTGCTGTGT	60	300 nM	[17]
	GACCAATACGGCATCTTCCTTC
TNFα	GGAATACCTGGACTATGCTGA	60	300 nM	This paper
	CCTCACTTCCCTACATCCCT
TGFβ	GGACATTAACGGGTTCAGTTC	57	200 nM	This paper
	GTCCAGGCTCCAGATGTAAG
Interferon γ	CTCCGGCCTAACTCTCTCCT	60	300 nM	[17]
	AGGCCCACCCTTAGCTACAT
GAPDH	GGTGATGCTGGTGCTGAGTA	60	300 nM	[18]
	TCATAAGTCCCTCCACGATG
SDHA	ACCTGATGCTTTGTGCTCTGC	60	200 nM	[18]
	CCTGGACGGGCTTGGAGTAA
G6PDH	CGAGGCTGTGTACACCAAGA	60	300 nM	[17]
	ATGTGGTGGAGCAGTGGAGT

ISSN: 1297-9716