Skip to main content

Table 1 Primer sequences used for qPCR experiments

From: Contribution of mammary epithelial cells to the immune response during early stages of a bacterial infection to Staphylococcus aureus

Genes Primers Sequence 5′ > 3′ Amplicon size, nt
αs2-casein (CSN1S2) forward CTG GTT ATG GTT GGA CTG GAA AA 76
  reverse AAC ATG CTG GTT GTA TGA AGT AAA GTG  
κ-casein (CSN3) forward AGG TGC AAT GAT GAA GAG TTT TTT C 66
  reverse CCC AAA AAT GGC AGG GTT AA  
Cluster of differentiation 3 epsilon (CD3e) forward ACG CTG TAC CTG AAA GCA AGA 118
  reverse AAT ACA CCA GCA GCA GCA AG  
Cluster of differentiation 68 (CD68) forward GAT CTG CTC TCC CTG AAG CTA CA 79
  reverse CAT TGG GAC AAG AGA AAC TTG GT  
Cluster of differentiation 18 (CD18) forward AGC GAC CTC AGG GAG TAC CA 65
  reverse TTA TCG TTG TTC CAC TGG GAC TT  
Chemokine 4 (CCL4) forward CAG CCG TGG TAT TCC AGA CC 109
  reverse CTC GGA GCA GCT CAG TTC AGT  
Cyclophilin A (PPAI) forward TGA CTT CAC ACG CCA TAA TGG T 62
  reverse CAT CAT CAA ATT TCT CGC CAT AGA  
Galectin 3 forward GTG GTA AAC CTT TCA AAA TAC AAG TGC 101
  reverse ATT TTT CAC CCG ATG ATT GTA CTG  
G protein-coupled receptor 97 (GPR97) forward GAG ATC ACC TTC TCC CAC CAG 204
  reverse TGT GGA GCA GCC CAA GGA  
Interleukin 8 (IL-8) forward TGA GAG TGG GCC ACA CTG C 103
  reverse CAC AAC CTT CTG CAC CCA CTT  
Keratin 14 (KRT14) forward CCC AGC TCA GCA TGA AAG C 57
  reverse AGC GGC CTT TGG TCT CTT C  
Serum amyloid A3 (SAA3) forward CTG GGC TGC TAA AGT GAT CAG TAA C 69
  reverse CCC TTG AGC AGA GGG TCT GTG ATT  
S100A12 forward TCC ACC AGT ACT CCA TCC GG 102
  reverse TGG TGT TTT TGA GGC AGT TGG  
Serglycin forward TCC AGC AGA ATC CCA CCT CTA 107
  reverse CCA GAA CCT GAT CCT GAG ACG  
SerpinA1 forward AAG AAA TAT GCA AGT TCT GCC AAT T 101
  reverse ACC CTG TTG ATG CCC AGT TC  
TNFα forward CAG AGG GAA GAG CAG TCC CC 101
  reverse TGG GCT ACC GGC TTG TTA TTT  
Toll-like receptor 2 (TLR2) forward TAA ACT TGA GAG TGG AGG TCA AAT CA 101
  reverse TCA GAG GCT CCT TCC GTG G  
Interleukin 1beta (IL-1β) forward GAC AAC AAG ATT CCT GTG GCC 101
  reverse TCT ACT TCC TCC AGA TGA AGT GT  
Pentraxin 3 (PTX3) forward CCG AGC TGT GCA GGG CT 101
  reverse GCA CGC TTG CAA AAA TCT TCT T  
Cathelicidin forward GAG AAT GGG CTG GTG AAA CAG 107
  reverse GGG CGA AGT CTC CTC ACA CTC  
24S ribosomal protein (RPS24) forward TGG TGGTGG CAA GAC AAC TG 66
  reverse TTC TTC GCG TAA TCC AAG GAA  
  1. Each pair of primers amplifies the target cDNA (amplicon sizes ranging between 57 and 248 nucleotides) in its 3′ UTR. Primer pairs were designed with primer Express Software v2.0 (Applied Biosystems) except for 24S ribosomal protein primers, which were manually designed.
Back to article page

Veterinary Research

ISSN: 1297-9716