Figure 2
From: Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate immune responses
Mycoplasma synoviae and its diacylated lipopeptide induce expression of TLR15 in primary chicken chondrocytes. A) Expression of TLR15, TLR1 and TLR2 mRNA after infection of cells with Mycoplasma synoviae. Primary chicken chondrocytes were infected with live Mycoplasma synoviae (MS) for 1 h, 6 h or 24 h and mRNA expression was analyzed by RT-qPCR. NC represents non-infected cells. Bars show mean ± S.E. (n = 4); ***, p < 0.001 compared to non-infected cells. B) Expression of TLR15, TLR1 and TLR2 mRNA after treatment of cells with diacylated lipopeptide or its non-acylated analog. Primary chicken chondrocytes were treated with 1 μM non-acylated peptide (NAP) or diacylated lipopeptide (MDLP) for 1 h, 6 h or 24 h and mRNA expression was analyzed by RT-qPCR. NC represents non-treated cells. Bars show mean ± S.E. (n = 4); **, p < 0.01 and ***, p < 0.001 compared to NAP-treated cells at each time point for selected gene.