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Table 2 Antibody pairs and standard ranges for bovine cytokines IFN-γ, IL-4, IL-10 and IL-12 ELISA

From: Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation

  Primary antibody Secondary antibody Standard range Reference
(Working concentration (μg/mL))
IFN-γ CC330 (5 μg/mL) CC302b (2 μg/mL) 5000 pg/mL –10 pg/mL  
IL-4 CC314 (5 μg/mL) CC313b (2 μg/mL) 125.00 U/mL -0.488 U/mL [32]
IL-10 CC318 (5 μg/mL) CC320b (2 μg/mL) 11 U/mL -0.021 U/mL [33]
IL-12 CC301 (1.25 μg/mL) CC326b (1 μg/mL) 86.42 U/mL -0.013 U/mL [34]