Maternal lymph node and spleen TLR-9 expression. Samples of maternal left prefemoral lymph node (LPF), right prefemoral lymph node (RPF), left uterine lymph node (LUL), right uterine lymph node (RUL), retropharyngeal lymph node (RLN) and spleen were collected at post mortem examination and snap frozen on dry ice. RNA was extracted and used to synthesise cDNA. Levels of expression of TLR-9 were examined with data being normalised against GAPDH expression (pg). The predicted mean responses (pg) (Infected -■-, Control -∆-) and 95% confidence intervals (error bars) of maternal spleen and lymph node on (A) 28 dpi, (B) 42 dpi, (C) 56 dpi.