Foetal lymph node and spleen IFN-γ responses following stimulation with NCA for 4 day. Foetal hepatic lymph node (HLN), mesenteric LN (MLN) and spleen samples were collected at post mortem examination. Following stimulation with NCA for 4 days (37° C in a humidified 5% CO2 atmosphere) cell free supernatants were harvested, ELISA were performed to determine the concentration of IFN-γ (ng/mL) produced. The data were log-transformed (base 10) before analysis by a linear model. The predicted mean responses (ng/mL on log10 scale) (Infected -■-, Control -∆-) and 95% confidence intervals (error bars) of foetal spleen and lymph node on (A) 14 dpi, (B) 28 dpi, (C) 42 dpi, (D) 56 dpi.