Levels of cytokines produced by splenic T cells from mice subcutaneously vaccinated with r Bsc -PYP-1. Splenocytes derived from trials I and II were stimulated with rBsc-PYP-1 (15 μg/mL) for 72 h in vitro, and concentrations of cytokines (IFN-γ, IL-2, IL-4 and IL-10) in the supernatant were measured by ELISA. Data are expressed as the mean ± SD for each group of 10 mice. Each sample was examined in triplicate. Asterisks indicate that the mean value was significantly higher than that of the group immunized with PBS plus FCA or PBS alone (*P < 0.01, **P < 0.001). NS denotes no statistically significant difference, and error bars indicate SD.