Table 1 Primer sequences and annealing temperatures for real-time PCR assays.
Gene | Genbank accession no. | Primer sequence (5′-3′) | TANN (°C) |
|---|---|---|---|
GAPDH [25] | AF030943 | F: GGT GAT GCT GGT GCT GAG TA | 57 |
| Â | Â | R: TCA TAA GTC CCT CCA CGA TG | Â |
IL-4 [28] | AF172168 | F: AGA GAT CAT CAA AAC GCT GAA | 55 |
| Â | Â | R: GTC TGC TAC AGG CAG CTC | Â |
IL-10 [25] | NM_001009327 | F: TGA AGG ACC AAC TGA ACA GC | 55 |
| Â | Â | R: TTC ACG TGC TCC TTG ATG TC | Â |
TGF-β1 [25] | NM_001009400 | F: GAA CTG CTG TGT TCG TCA GC | 55 |
| Â | Â | R: GGT TGT GCT GGT TGT ACA GG | Â |
IFN-γ [27] | NM_001009803 | F: CTA AGG GTG GGC CTC TTT TC | 55 |
| Â | Â | R:CAT CCA CCG GAA TTT GAA TC | Â |