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Table 1 Primer sequences and annealing temperatures for real-time PCR assays.

From: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

Gene Genbank accession no. Primer sequence (5′-3′) TANN (°C)
GAPDH [25] AF030943 F: GGT GAT GCT GGT GCT GAG TA 57
   R: TCA TAA GTC CCT CCA CGA TG  
IL-4 [28] AF172168 F: AGA GAT CAT CAA AAC GCT GAA 55
   R: GTC TGC TAC AGG CAG CTC  
IL-10 [25] NM_001009327 F: TGA AGG ACC AAC TGA ACA GC 55
   R: TTC ACG TGC TCC TTG ATG TC  
TGF-β1 [25] NM_001009400 F: GAA CTG CTG TGT TCG TCA GC 55
   R: GGT TGT GCT GGT TGT ACA GG  
IFN-γ [27] NM_001009803 F: CTA AGG GTG GGC CTC TTT TC 55
   R:CAT CCA CCG GAA TTT GAA TC  
  1. TANN = primer annealing temperature.