Figure 4

BAG3 down-regulation induces apoptosis and affects cell cycle progression in EqS04b cells. (a) On the Y axis, the percentages of hypodiploid cells (subG₀/G₁) and of cells in each cell cycle phase of BAG3siRNA-transfected EqS04b cells subtracted for the corresponding percentages in scrRNA-transfected cells. Data presented are the mean values ± S.E.M. from at least three experiments (*p < 0.05, **p < 0.001 calculated on raw data of BAG3siRNA-silenced versus scrRNA-treated cells). Mean values (%) ± S.E.M. in scrRNA-transfected cells: i) at 36 h, subG₀/G₁ 4.9 ± 1.2; G₀/G₁ 68.59 ± 3.7; S 21.08 ± 1.8; G₂/M 9.03 ± 1.0; ii) at 56 h, subG₀/G₁ 3.5 ± 1; G₀/G₁ 66.8 ± 2.3; S 22.4 ± 2.1; G₂/M 10.6 ± 1.1. Representative histograms of cell cycle profiles of scrRNA- and BAG3siRNA-transfected cells at 36 h are on the left. (b) On the Y axis, the percentages of Annexin V positive (A⁺/PI^-, early apoptotic cells; A⁺/PI⁺, late apoptotic cells) and necrotic cells (A^-/PI⁺) in BAG3siRNA-silenced EqS04b cells subtracted for the corresponding percentages in scrRNA-transfected cells. Data presented are the mean values ± S.E.M. from at least three experiments (*p < 0.05, **p < 0.001 calculated on raw data of BAG3siRNA-silenced versus scrRNA-treated cells). Mean values (%) ± S.E.M. in scrRNA-transfected cells: i) at 36 h, A⁺/PI^-, 4.2 ± 1.1; A⁺/PI⁺, 2.3 ± 0.96; A^-/PI⁺, 3.1 ± 1; ii) at 56 h the values became comparable to those of non-transfected control cells (≤ 2% in A⁺/PI^-, A⁺/PI⁺, A^-/PI⁺ gated quadrants).