Figure 2
Examples of different immune cell infiltration in placentomes from N. caninum -inoculated animals. (a) Severe infiltration of macrophages in the base of maternal caruncle of a N. caninum inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 100×. (b) Mild aggregate of T helper cells (CD4+) surrounding a blood vessel in maternal caruncle of a placentome collected from an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (c) Minimal infiltration of cytotoxic T-cells (CD8+) in a necrotic focus in maternal caruncle of a N. caninum challenged cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (d) Mild aggregate of γδ T-cells in the base of maternal caruncle of an inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 200×. (e) Rare presence of NK-cells in connective tissue in the maternal caruncle surrounding necrotic and mineralized foetal villi, observed in a placenta of an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (f) Rare presence of CD79αcy+ cells aligned with endometrial epithelial cells in the maternal caruncle and in some foetal villi, observed in the placentome of a N. caninum inoculated cow culled 28 dpi. Original magnification 400×. Counterstained with haematoxylin.