Recombination of FIPV from cats 1, 7, 9, 10, 11, 12 and 13 on the S gene. The alignment of the 3′ end of the S gene to the downstream genes of FCoV isolated from seven FIP cats with type I FCoV and CCoV. The light and dark shaded areas encompass higher similarity to CCoV and type I FCoV, respectively. The putative recombination event occurred at nucleotide 4250 based on the comparison with FCoV NTU2 and is indicated with an arrow. The sequences were obtained from FIPV found in individual samples and tissues and are shown collectively. NOC: nasal/oral/conjunctival swabs; RS: rectal swabs; As: ascites; PE: pleural effusion; Li: liver; Lu: lung; Ki: kidney; Br: brain; Sp: spleen; dbd: days before death. GenBank accession number: FCoV C1Je (GenBank: DQ848678), FCoV Black (GenBank: EU186072), FCoV NTU2 (GenBank: DQ160294) and CCoV NTU336 (GenBank: GQ477367).