Transcriptional analysis of CyHV-3 ORF134 genome region. CCB cells were infected with the indicated recombinant strains at a MOI of 0.5 PFU/cell. Twenty-four hours post-infection, expression of CyHV-3 ORF55, ORF132, ORF133, ORF134, ORF135 and carp β-actin was studied by the RT-PCR approach described in the Materials and methods. On the left RT-PCR or PCR represent PCR products generated when the RT was performed or omitted from the reactions, respectively. On the right, control PCR reactions were performed using genomic DNA as template (Ctrl+) or no template (Ctrl-).