Cell death in MDM from resistant or susceptible deer following in vitro MAP infection. (A) Detection of apoptosis by TUNEL TMR red staining of MDM from crossbred animals of resistant or susceptible genotype at 24 or 48 h after MAP infection. Results are shown as mean percentage TUNEL positive cells ± SEM, n = 8 (4 resistant and 4 susceptible). Un = untreated, MAP = MAP-infected, STS = staurosporine at 200 ng/mL for 6 h (positive control). (B) Detection of total cell death by LDH release from MDM isolated from crossbred animals of resistant or susceptible genotype. Following 24 or 48 h MAP infection, supernatants of cultures were collected and analysed for LDH release. Results are given as mean absorbance values ± SEM, n = 13 (6 resistant and 7 susceptible).