Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice

Table 1 The PCR primers used for detection and qPCR of transgenic cells and mice

Name	Sequence	Function
GFP-F	GGTCTTGTAGTTGCCGTCGT	Quantitate GFP in BHK-21 cells
GFP-R	TCGTGACCACCCTGACCTAC	Quantitate GFP in BHK-21 cells
h-actin-F	CACGCAGCTCGTTGTAGAAG	Quantitate β-actin in BHK-21 cells
h-actin-R	AGGACTCGTACGTGGGTGAC	Quantitate β-actin in BHK-21 cells
PBL1-F	CAACATACGAGCCGGAAGCATAAA	Detect PBL in cell lines
PBL1-R	TAACAGAATCTTGACCTTGCCACA	Detect PBL in cell lines
PBR1-F	AGTCAGTCAGAAACAACTTTGGCACA	Detect PBR in cell lines
PBR1-R	ACGAATAGGTGGCCTATGGCATTATTG	Detect PBR in cell lines
PBase-F	GCCACCATGGGATGTTCTTTAG	Detect transposase in cell lines
PBase-R	GTACTCAGAAACAACTTTGGC	Detect transposase in cell lines
Q-PBR1-F	TACGCATAAACGATGACG	Quantitate copy number in cell lines
Q-PBR1-R	CAAACTAAAGGCGGAGTG	Quantitate copy number in cell lines
Neo-2B-F	TGACCGCTTCCTCGTGCTTT	Detect transgenic mice
Neo-2B-R	TGGGTGTTCCCGCCTAGTGA	Detect transgenic mice
3D-Neo-F	ATCCGACGCCGCCATCTCTA	Detect transgenic mice
3D-Neo-R	CGGAGAACCTGCGTGCAATC	Detect transgenic mice
NR-F	CGTTTCGCATGATTGAACAAGAT	Quantitate copy number in mice
NR-R	GTGCCCAGTCATAGCCGAAT	Quantitate copy number in mice
GI-F	GGTGGTGAATACCATGTACAAAGCT	Quantitate GAPDH as reference in mice
GI-R	CCAACACCCCCAGTCATACG

ISSN: 1297-9716