The synergistic necrohemorrhagic action of Clostridium perfringens perfringolysin and alpha toxin in the bovine intestine and against bovine endothelial cells

Table 1 Description of strains and toxin activities in supernatants of an overnight culture

Strain^a	Strain number	Relevant characteristics^b	Plasmid-encoded toxin gene(s)^a	Plc activity(μg mL^-1)^c	PFO activity (log₂(titre))^c	Ref.
Wild-type	JIR325	Strain 13^d		12.5 ± 2.5	4.8 ± 0.2	[25]
ΔpfoA Δplc	JIR4444	JIR325 pfoA::ermB plcΩpJIR1774, suicide plasmid		< 1.0	< 1.0	[23]
pfoA-complemented ΔpfoA Δplc	JIR4460	JIR4444(pJIR871), Cm^R	pfoA ⁺	< 1.0	4.1 ± 0.3	[23]
plc-complemented ΔpfoA Δplc	JIR4461	JIR4444(pJIR1642), Cm^R	plc ⁺	6.8 ± 0.7	< 1.0	[23]
double-complemented ΔpfoA Δplc	JIR4462	JIR4444(pJIR1720), Cm^R	pfoA⁺plc⁺	8.0 ± 0.5	3.5 ± 0.1	[23]

^apfoA: perfringolysin gene, plc : alpha toxin gene, ^bCm^r: chloramphenicol resistant, ^cPlc : alpha toxin, PFO: perfringolysin O, ^dJIR325 is a rifampicin and nalidixic acid resistant derivative of strain 13, a gas gangrene strain.

ISSN: 1297-9716