Analysis by RT-qPCR of the expression of a set of genes showing differential expression in the microarray analysis. Relative quantification of the TNFa, IL6, Ccl2, Ccl5, Ccl20, Nos2 and CD83 transcripts by RT-qPCR 3 h or 6 h after stimulation of bMEpC with LPS or SaS. Median values (Q1 and Q3) from bMEpC of five cows are shown. * Statistical significance relative to Control. § Statistical significance, LPS versus SaS.