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Table 2 Virus isolation and nucleic acid detection in samples obtained from organs and cloacal swabs

From: Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Group

dpi

Cloacal swabs

Gizzard

Liver

Spleen

VI

Real-time PCR

VI

Real-time PCR

VI

Real-time PCR

VI

Real-time PCR

SPFB

3

5/5 a

5/5

5/5

5/5

4/5

5/5

0/5

NE b

7

5/5

5/5

5/5

5/5

5/5

5/5

4/5

5/5

10

5/5

5/5

5/5

5/5

5/5

5/5

1/5

2/5

14

0/5

5/5

0/5

4/5

0/5

5/5

0/5

NE

17

0/5

5/5

0/5

3/5

0/5

3/5

0/5

3/5

CB

3

5/5

5/5

3/5

5/5

0/5

3/5

0/5

NE

7

4/5

5/5

5/5

5/5

1/5

5/5

0/5

4/5

10

5/5

5/5

5/5

5/5

2/5

5/5

0/5

4/5

14

4/5

5/5

1/5

5/5

0/5

4/5

0/5

NE

 

17

0/5

5/5

1/5

5/5

0/5

5/5

0/5

1/5

  1. Samples from specific-pathogen-free (SPFB) and commercial (CB) broilers orally infected with a virulent FAdV-1 were investigated at 3, 7, 10, 14 and 17 days post infection (dpi) by virus isolation (VI) and/or real-time PCR.
  2. a No. of positive samples/no. of tested samples.
  3. b NE – not examined.