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Table 2 Virus isolation and nucleic acid detection in samples obtained from organs and cloacal swabs

From: Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Group dpi Cloacal swabs Gizzard Liver Spleen
VI Real-time PCR VI Real-time PCR VI Real-time PCR VI Real-time PCR
SPFB 3 5/5 a 5/5 5/5 5/5 4/5 5/5 0/5 NE b
7 5/5 5/5 5/5 5/5 5/5 5/5 4/5 5/5
10 5/5 5/5 5/5 5/5 5/5 5/5 1/5 2/5
14 0/5 5/5 0/5 4/5 0/5 5/5 0/5 NE
17 0/5 5/5 0/5 3/5 0/5 3/5 0/5 3/5
CB 3 5/5 5/5 3/5 5/5 0/5 3/5 0/5 NE
7 4/5 5/5 5/5 5/5 1/5 5/5 0/5 4/5
10 5/5 5/5 5/5 5/5 2/5 5/5 0/5 4/5
14 4/5 5/5 1/5 5/5 0/5 4/5 0/5 NE
  17 0/5 5/5 1/5 5/5 0/5 5/5 0/5 1/5
  1. Samples from specific-pathogen-free (SPFB) and commercial (CB) broilers orally infected with a virulent FAdV-1 were investigated at 3, 7, 10, 14 and 17 days post infection (dpi) by virus isolation (VI) and/or real-time PCR.
  2. a No. of positive samples/no. of tested samples.
  3. b NE – not examined.