Combined analysis of CD8α, CD27, CD45RC and SLA-DR expression on T helper cells. Boolean gating was applied for a linked analysis of the four markers within blood (PBMCs), spleen, mediastinal (Med. LN), bronchial (Bron. LN) and mesenteric lymph nodes (Mes. LN), tonsil, and liver. Results for five animals are shown in pie charts. The table defines the colour code that was used to differentiate the sixteen different subpopulations which result from expression/non-expression of the four markers CD8α, CD27, CD45RC and SLA-DR. Data are derived from FCM analyses described in Figure 1. Only CD3+CD4+ T helper cells were analysed by boolean gating.