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Figure 1 | Veterinary Research

Figure 1

From: Immunogenic and protective properties of GP5 and M structural proteins of porcine reproductive and respiratory syndrome virus expressed from replicating but nondisseminating adenovectors

Figure 1

Expression of recombinant adenoviruses (rAdV) in vitro. Western blot analysis of A549 cell lysates from mock infected (lane 1) or infected with rAdV (MOI of 1) expressing GFP (lane 2), M-GP5 (lane 3) and M-GP5m (lane 4). Immunoblot was performed using GFP-specific mouse monoclonal antibody or a convalescent PRRSV-specific pig antiserum as primary antibody and an anti-mouse or anti-pig IgG-HRP as secondary antibody. Replication capability of rAdV was confirmed by E1A expression (lanes 2–4). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) immunostaining was used as a loading control.

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Veterinary Research

ISSN: 1297-9716