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Table 3 Transposon insertion sites of M. hyopneumoniae strain 232 mutants.

From: Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

Mutant Transposon insertion location ORF disrupted Gene function
72 84920 Mhp069 Unknown
11 85345 Mhp069 Unknown
40 259306 Tig Trigger factor
159 275721 NCR Unknown
50B 284811 Mhp261 Unknown
4 299711 Mhp271 P97 ciliary adhesion
96 318725 P95 Outer membrane protein
165 318913 P95 Outer membrane protein
91 359210 Mhp313 Unknown
9 434381 Mhp366 Unknown
34 466299 ulaA PTS system ascorbate-specific transporter subunit IIC
37 522533 Mhp443 Unknown
54B 527019 Mhp445 Unknown
156 537514 Mhp446 Unknown
75 540819 Mhp447 Unknown
53B 541339 Mhp447 Unknown
102 542436 Mhp447 Unknown
90 542541 Mhp447 Unknown
1 544071 Mhp447 Unknown
78 544309 Mhp447 Unknown
24 544767 Mhp447 Unknown
5 545264 Mhp447 Unknown
68 549968 Mhp447 Unknown
49 549968 Mhp447 Unknown
161 549968 Mhp447 Unknown
52B 551617 NCR Unknown
13 552503 NCR Unknown
56 574428 NCR Unknown
66 574428 NCR Unknown
17 608509 Mhp490 PTS system fructose-specific transporter subunit IIABC
16 652232 pepF Oligoendopeptidase F
162 653224 pepF Oligoendopeptidase F
101 653333 pepF Oligoendopeptidase F
92 653430 pepF Oligoendopeptidase F
47 672237 Mhp535 Unknown
170 672318 Mhp536 Unknown
63 672979 Mhp537 Unknown
50 686279 Mhp542 Unknown
19 722926 ulaA PTS system ascorbate-specific transporter subunit IIC
2 759509 NCR Regulatory region of glpD
10 788260 Mhp630 Unknown
7 788457 Mhp630 Unknown
12 790862 Mhp631 Unknown
51B 856887 pepP xaa-pro aminopeptidase
  1. The locations of transposon insertions within the M. hyopneumoniae strain 232 genome for 44 individual pMHC9-1 transformants are shown. The open reading frame (ORF) or non-coding region (NCR) containing the insertion is shown and, where known, the function of the protein encoded.