Skip to main content

Table 1 Sequences of oligonucleotides.

From: Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

Oligonucleotide

Restriction sites

Oligonucleotide sequence (5’-3’)

TetM1

Pst I

GCCGCTGCAG AATTAAAAGTTAGTG

TetM2

Xba I

GAAATCTAGA TTATATAACAACTTAAATTAC

TetM3

Sbf I

CGATCCTGCAGG CAGAATTAAAAGTTAGTG

TetM4

Mlu I

CAGTACGCGT TTATATAACAACTTAAATTAC

Tpn1

Nde I/NcoI

GCGCATATGCCATGG AAAAAAAGGAATTTCGTG

Tpn2

Xho I

CGCCACTCGAG ATTATTCAACATAGTTCCCTTC

P97-1

Nde I

CGATCATATG ACGGGGATTTAAAACAGAAAC

P97-2

Nco I

GAATCCATGG CACCAACAATTCCGGCAGTC

TetMF

N/A

GTGGACAAAGGTACAACGAG

TetMR

N/A

CGGTAAAGTTCGTCACACAC

Linker-A

N/A

CGACTGGACCTGGA

Linker-B

N/A

GATAAGCAGGGATCGGAACCTCCAGGTCCAGTCG

L-PCR-C

N/A

GATAAGCAGGGATCGGAACC

L-PCR-L

N/A

GATAGGGTTGAGTGTTGTTC

L-PCR-R

N/A

TAGTCGGATAGATAAAGTAC

  1. Sequences of oligonucleotides used in the study are shown. Where appropriate, the restriction enzyme recognition sites added to the 5’ end of the oligonucleotides are underlined.
Back to article page

Veterinary Research

ISSN: 1297-9716