Salmonella Typhimurium labeling and gene expression in porcine MLN. A-B: Different labeling profiles found for Salmonella Typhimurium in porcine MLN. Scale bar = 10 μm. (A) Pathogen detection as spherical structures in the perinuclear zone of mononuclear cells. (B) Staining of bacilli shaped structures. C: Analysis of Salmonella Typhimurium gene expression by SCOTS in vivo and in vitro. Black dots and bars respectively represent individual and mean expression values from analysis of cDNA from pig infected MLN. Triangles (early logarithmic phase) and squares (late logarithmic phase) denote gene expression data from Salmonella Typhimurium cultures. Higher values mean higher expression levels and vice-versa.