Analysis of MHC molecules in porcine MLN after Salmonella Typhimurium infection. A: Western blot analysis of MHC class I and II at 1, 2 and 6 dpi. B-E: MHCII labeling in tissue at 0 (B), 1(C), 2 (D) and 6 (E) dpi by immunohistochemistry. Scale bar = 20 μm. F-G: mRNA quantification of SLA-DRB5 (MHCII) (F) and SLA-B (MHCI) (G) by qPCR. H-K: confocal analysis of Salmonella Typhimurium infected MLN demonstrate the presence of the pathogen in MHCII positive cells. Salmonella Typhimurium- FITC (H), MHCII-Alexa Fluor® 594 (I), DAPI (J) and merge (K).