FMDV-induced and immune complexe-induced IFN-α occurs through a TLR7-dependant pathway. A. IFN-α responses of enriched pDC (CD14-depletion/CD172a-enrichment) stimulated for 16 h with influenza A virus (PR8) in absence or presence of different IRS661 (TLR7 inhibitor) or control ODN (Ctrl ODN) concentrations. B. Impact of IRS661 (0.7 μM) on IFN-α responses of enriched pDC stimulated with FMDV O UKG 2001 (MOI of 1 TCID50/cell) pre-incubated for 30 min with naïve and anti-O Manisa sera. After 24 h, IFN-α was quantified by ELISA. The mean value and the standard deviations of triplicates cultures are shown. The data are representative of two independent experiments.