Figure 5

Relative cell counts of CD4⁺CD8⁻T helper cells (T _H ) and CD4⁺CD25^hiT regulatory helper (T _reg ) cell subpopulation in piglets of the probiotic ( n = 6 per time point, grey boxes) and the control group ( n = 6 per time point, white boxes) at different days of life. Cell counts are given either for T_H cells as counts relative to the total number of living lymphocytes or for the T_regs as counts relative to the total number of T_H cells. A) and B) show the flow cytometry dot plots of one representative piglet. The marked population was considered as A) T_H and B) T_reg cells. C) T_H cells and D) T_reg cells in ileal lymph nodes (IL LN). E) T_H cells and F) T_reg cells in continuous ileal Peyer’s Patch (IL PP). A t-test indicated significant differences between the feeding groups in the ileal lymph nodes at 54 days of age (*0.01 < p < 0.05).