Relative cell counts of CD4+CD8−T helper cells (T
) and CD4+CD25hiT regulatory helper (T
) cell subpopulation in piglets of the probiotic ( n = 6 per time point, grey boxes) and the control group ( n = 6 per time point, white boxes) at different days of life. Cell counts are given either for TH cells as counts relative to the total number of living lymphocytes or for the Tregs as counts relative to the total number of TH cells. A) and B) show the flow cytometry dot plots of one representative piglet. The marked population was considered as A) TH and B) Treg cells. C) TH cells and D) Treg cells in ileal lymph nodes (IL LN). E) TH cells and F) Treg cells in continuous ileal Peyer’s Patch (IL PP). A t-test indicated significant differences between the feeding groups in the ileal lymph nodes at 54 days of age (*0.01 < p < 0.05).