Profiles of PrPdaccumulation in the brain of inocula donors. A, B: AAS: note the high degree of similarity between the individual profiles (A), which are characterized by prominent extracellular deposits, particularly those associated to glial cells (A type; sub-pial, stellate, peri-vascular and perivacuolar, as illustrated in B; inset, detail of peri-vascular and peri-vacuolar aggregates in white matter of the cerebral cortex). C, D: VVC: note slight more variable and clearly different profiles (C) than in AAS donors, with prominence of vascular plaques in cerebral cortex (X type, as illustrated in D; inset, detail of intramural and perivascular plaques in corpus striatum). IHC with R145 PrP monoclonal antibody and haematoxylin counterstaining. Original magnifications: large images × 4, insets × 20). PrPd types (X-axis) are: ITNR: intraneuronal; ITGL: intraglial; GLAS: glia-associated extracellular; NRPL: extracellular in grey matter neuropil; OTHR: other types. For more detailed description see text. Y-axis indicates proportion of the different PrPd types.