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Table 1 Solvent systems for TLC analysis of mycobacterial lipids (adapted from[22]).

From: Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells

Solvent System Run Direction Components Runs Lipids Resolved
A 1 petroleum ether : ethyl acetate (98 : 2) 3 PDIM, TAG, MQ
  2 petroleum ether : acetone (98 : 2) 1
B 1 petroleum ether : acetone (92 : 8) 3 AT, FA
  2 toluene : acetone (95 : 5) 1
C 1 chloroform : methanol (96 : 4) 1 FA, GLY
  2 toluene : acetone (80 : 20) 1
D 1 chloroform : methanol : water (100 : 14 : 0.8) 1 CF, SL, DAT
  2 chloroform : acetone : methanol : water (50 : 60: 2.5 : 3) 1
E 1 chloroform : methanol : water (60 : 30 : 6) 1 DPG, PE PI, PIM
  2 chloroform : acetic acid : methanol : water (40 : 25 : 3 : 6) 1